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Sensitization to the major allergen of Brazil nut is correlated with the clinical expression of allergy  Elide A. Pastorello, MDa, Laura Farioli, BScb,

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Presentation on theme: "Sensitization to the major allergen of Brazil nut is correlated with the clinical expression of allergy  Elide A. Pastorello, MDa, Laura Farioli, BScb,"— Presentation transcript:

1 Sensitization to the major allergen of Brazil nut is correlated with the clinical expression of allergy  Elide A. Pastorello, MDa, Laura Farioli, BScb, Valerio Pravettoni, MDa, Marco Ispano, MDc, Amedeo Conti, BScd, Raffaella Ansaloni, MDc, Federica Rotondo, MDc, Cristoforo Incorvaia, MDa, Anders Bengtsson, BSce, Federica Rivolta, MDa, Chiara Trambaioli, BSca, Mario Previdi, MDb, Claudio Ortolani, MDc  Journal of Allergy and Clinical Immunology  Volume 102, Issue 6, Pages (December 1998) DOI: /S (98) Copyright © 1998 Mosby, Inc. Terms and Conditions

2 Fig. 1 SDS-PAGE of raw Brazil nut extract stained with Coomassie Brilliant Blue. Protein content was mg × 1 cm of gel; because this lane was 2.5 cm, the protein content of the gel in the figure was 0.21 mg. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

3 Fig. 2 IgE immunoblotting of raw Brazil nut extract, with 25 μg of Brazil nut extract loaded for every strip, in sera from 11 symptomatic patients and 10 asymptomatic patients. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

4 Fig. 3 IgE immunoblot of the Brazil nut extract with pooled sera from symptomatic patients (lane 1) . The same pooled serum was used to detect 9-kd purified protein (lane 2) and inhibition of raw Brazil nut extract by 9-kd purified protein at different protein concentrations (lanes 3 and 4) . Negative serum was used for the IgE blot of the raw extract to detect aspecific binding (lane 5) . Lane 6 shows IgE blot of raw extract with pooled sera from asymptomatic patients under investigation. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

5 Fig. 4 Chromatographic profile of raw Brazil nut extract separated on Superdex 75 HPLC column by size-exclusion chromatography (top) . SDS-PAGE of raw brazil nut extract (left lane) and of purified 2S albumin (right lane) stained with Coomassie Brilliant Blue (bottom) . Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

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