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Fig. 3. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Immunostaining for.

Published byPentti Lahtinen Modified over 5 years ago

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Presentation on theme: "Fig. 3. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Immunostaining for."— Presentation transcript:

1 Fig. 3. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Control (A,C,E,G,I,K) and Lhx1-overexpressing eyes (B,D,F,H,J,L). In these experiments, a bicistronic vector of pCAGGS-RFP-2A-Lhx1 was used. (A,B) 3A10, (C,D) N-cadherin, (E,F) HuC/D RNA-binding protein, (G,H) Islet1, (I,J) visinin (calcium-binding protein), and (K,L) AP2α. N-cadherin is localized to the neuroepithelium, visinin is localized to future cone photoreceptors, and others are localized to early retinal ganglion cells, and AP2α is localized to differentiating amacrine cells. Note that induced neural retina (iNR) is thicker than the authentic NR in this experimental condition. Scale bar: 100 µm. Takumi Kawaue et al. Biology Open 2012;1: © Published by The Company of Biologists Ltd

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