1. Ondřej Zítka Datum konání: 20.11.2015
Electrochemical detection of lung cancer specific microRNAs using 3D DNA origami nanostructures
Ondřej Zítka
Datum konání:
Investice do rozvoje vzdělávání

2. Background
Recent reports have indicated that aberrant expression of microRNAs is highly correlated with occurrence of lung cancer.
Therefore, highly sensitive detection of lung cancer specific microRNAs provides an attractive approach in lung cancer early diagnostics.

3. The probe for sensor
The 3D DNA origami structure is constituted of a ferrocene-tagged DNA of stem-loop structure combined with a thiolated tetrahedron DNA nanostructure at the bottom.
The top portion hybridized with the lung cancer correlated microRNA, while the bottom portion was self-assembled on gold disk electrode surface, which was modified with gold nanoparticles (Au NPs) and blocked with mercaptoethanol (MCH).

4. Scheme of the genosensor

5. Characterisation of sensor
The preparation process and the performance of the proposed electrochemical genosensor were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

6. Characterisation of sensor
(A) CV curves o fgold disk electrodes before (b) and after (a) deposition of gold nanoparticles in 0.5M H2SO4 from 0 to1.5V at a scan rate of100mV/s.(B) SEM image for the AuNPs-coatedgoldelectrode. AFM images for the (C) bare,(D) AuNPs-coatedand (E) 3D DNA probe/Au NPs-coated gold electrodes.

7. Performance of electrochemical genosenzor
(A) DPV for the electrochemical genosensor in the presence of target mi-croRNAs at 1mM,100nM,10nM,1nM,100pM,10pM and 0pM (from top to bottom).
(B) The linear relationship between peak current increasement and microRNA concentration.
(C) DPV curves of electrochemical genosensor applied in 1 mM microRNA dissolved in RNAse-free PBS(b) and cell lysis(a).

8. Summary
Under the optimal conditions, the developed genosensor had a detection limit of 10 pM and a good linearity with microRNA concentration ranging from 100 pM to 1 µM, which showed a great potential in highly sensitive clinical cancer diagnosis application.

9. Investice do rozvoje vzdělávání
Acknowledgements:
Project:
Paper:
Budování výzkumných týmů a rozvoj univerzitního vzdělávání výzkumných odborníků pro mikro- a nanotechnologie (NANOTEAM) CZ.1.07/2.3/.00/
Liu, S.P., Su, W.Q., Li, Z.L. and Ding, X.T. (2015) Electrochemical detection of lung cancer specific microRNAs using 3D DNA origami nanostructures. Biosensors & Bioelectronics, 71,
Investice do rozvoje vzdělávání