1. Representative polyacrylamide/6% urea gels that demonstrate transferrin saturation (TSAT) for (A) a patient who was given the low-dosage (1.5 mg/kg) infusion and (B) a patient who was given the high-dosage (3.0 mg/kg) infusion.
Representative polyacrylamide/6% urea gels that demonstrate transferrin saturation (TSAT) for (A) a patient who was given the low-dosage (1.5 mg/kg) infusion and (B) a patient who was given the high-dosage (3.0 mg/kg) infusion. The lanes are identified with the time after start of infusion. The various transferrin species based on iron saturation are indicated. The higher band for monoferric transferrin is iron bound to the C terminus, and the lower band is the N terminus site bound to iron. X, artifact or contaminant and is not transferrin. At 60 min (Tmax for total iron), there is little change from baseline with either dosage. At 6 h after the high dosage, (Tmax for transferrin-bound iron), almost all of the transferrin is diferric. By 48 h, the distribution is back to baseline. Whereas a majority of patients who received high-dosage infusion showed similar qualitatively “total” saturation at 6 h, very few of the patients who received the low-dosage infusion did so.
Bradley A. Warady et al. CJASN 2007;2:
©2007 by American Society of Nephrology