1. SCAP and SREBP-2 are important for synapse formation, nerve firing, and memory tasks.
SCAP and SREBP-2 are important for synapse formation, nerve firing, and memory tasks. Top: SREBP-2 was silenced with a green fluorescent protein (GFP)-lentiviral construct (Lenti-shSREBP-2 [short hairpin SREBP-2]) in primary cultured mouse hippocampal cells. The PSD95 marker is represented in red, and the neuronal marker MAP2 is shown in blue. Adapted from Suzuki et al. (61). Middle: Mice with heterozygous deletion of SCAP show a decrease in spontaneous neuronal activity, as determined by basal recordings of miniature excitatory postsynaptic current in area CA1 neurons (adapted from Suzuki et al. [99]). Bottom: Impaired memory task performance. For the novel object recognition test, mice were trained for 5 min with two identical objects. One hour later, they were placed back in the same cage with one familiar and one novel object, and were monitored by video for 5 min. Mice with a heterozygous deletion of SCAP in the brain showed no exploratory preference for the novel object. Adapted from Suzuki et al. (99). CTR, control.
André Kleinridders et al. Diabetes 2014;63:
©2014 by American Diabetes Association