1. (A and B) Unweighted (A) and weighted (B) UniFrac clustering of three 16S rRNA hypervariable regions using fragment insertion SATé-enabled phylogenetic placement (SEPP) technique.
(A and B) Unweighted (A) and weighted (B) UniFrac clustering of three 16S rRNA hypervariable regions using fragment insertion SATé-enabled phylogenetic placement (SEPP) technique. (C) Box plot of the Shannon diversity indices between 16S rRNA V1-V2, V3-V4, and V4 regions. Statistical significance was calculated using Kruskal-Wallis test. (D) Relative abundances (at the order level) of gut microbiota showing statistically significant differences between 16S rRNA V1-V2, V3-V4, and V4 regions. Taxa in red show statistical significance according to nonparametric Wilcoxon signed-rank test (P < 0.05) (see details in Table S4).
Zigui Chen et al. mSystems 2019; doi: /mSystems