1. Biphasic ambulatory activity and reduced avoidance in Id2−/− mice.
Biphasic ambulatory activity and reduced avoidance in Id2−/− mice. (A–C) Spontaneous locomotor activity in wild-type (WT) and Id2−/− mice (n=8 mice per group) was analyzed using the open-field test at 1, 3 and 6 months of age (video-tracking, analyzed using Anymaze software). Changes in distance travelled (A), average speed (B) and total body rotations (C) were analyzed using two-way repeated measure ANOVA. Changes at individual time points were analyzed using post-hoc Bonferroni-corrected t-test for multiple comparisons; number of comparisons, 3. (D) Representative track plot tracings generated by Anymaze software demonstrate ambulatory activity in wild-type and Id2−/− mice at 1, 3 and 6 months of age. (E,F) Crossings into, and time spent in, an identically defined center zone were quantified from the same cohort of animals analyzed in A–C. Total entries (E) and time spent in the center zone (F) were analyzed as for A–C. (G,H) Avoidance behaviors were analyzed in a naïve cohort of wild-type and Id2−/− mice (n=6 mice per group) using the elevated plus maze paradigm. Entries (G) and time spent (H) in open arms (OA) and closed arms (CA) were calculated from track plots using Anymaze software. All data are expressed as the average of each group; error bars represent s.e.m. *P<0.05 analyzed using a two-tailed t-test for effect resulting from genotype in each measure.
Matthew C. Havrda et al. Dis. Model. Mech. 2013;6:
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