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Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice  Masayuki.

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Presentation on theme: "Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice  Masayuki."— Presentation transcript:

1 Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice  Masayuki Kaneko, MS*, Andrew Schimming, BA, Gerald J. Gleich, MD, Hirohito Kita, MD  Journal of Allergy and Clinical Immunology  Volume 105, Issue 6, Pages (June 2000) DOI: /mai Copyright © 2000 Mosby, Inc. Terms and Conditions

2 Fig. 1 Kinetics of changes in neutrophil (left) and eosinophil (right) numbers in peritoneal cavities of mice after allergen challenge. BALB/c mice were actively sensitized with ragweed extract (RW) and challenged by injecting the ragweed extract (filled circles) or saline control (open circles) intraperitoneally. Peritoneal lavage fluids were collected at indicated time points, and neutrophil and eosinophil cell numbers were counted. Results are shown as means ± range of two mice at each time point. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

3 Fig. 2 Neutrophil (left) and eosinophil (right) numbers in peritoneal cavities of mice 8 or 24 hours after allergen challenge. BALB/c mice were actively sensitized with ragweed (RW) extract and challenged by injecting the ragweed extract (filled columns) or saline control (open columns) intraperitoneally. Peritoneal lavage fluids were collected 8 or 24 hours after challenge, and neutrophil and eosinophil numbers were counted. Results are shown as means ± SEM from 4 to 6 mice in each group. *Significantly different from control (P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

4 Fig. 3 Immediate reaction of double-sensitized mice challenged with ragweed extract (RW) or DNP-OVA. All mice were actively sensitized by subcutaneous injection of ragweed extract for 20 days and passively sensitized by intravenous injection of anti-DNP mouse IgE (30 μg/kg) 24 hours before challenge; control mice were sensitized only with ragweed extract. Mice were challenged intraperitoneally by injection with either sterile 0.15 mol/L NaCl, ragweed extract, or DNP-OVA (DNP) at 30 μg/mL. Immediate responses (A, histamine; B, vascular permeability) were measured 10 minutes after the challenge, as described in the “Methods” section. Results are shown as means ± SEM from 3 to 6 experiments. *Significantly different from mice challenged with 0.15 mol/L NaCl (P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

5 Fig. 4 LPR (at 48 hours) in double-sensitized mice challenged with ragweed extract or DNP-OVA. All mice were actively sensitized by subcutaneous injection of ragweed extract (RW) for 20 days and passively sensitized by intravenous injection of anti-DNP mouse IgE (30 μg/kg) 24 hours before challenge; control mice were sensitized only with ragweed extract. Mice were challenged intraperitoneally by injection with either sterile 0.15 mol/L NaCl, ragweed extract, or DNP-OVA (DNP) at 30 μg/mL. LPRs (A, total leukocyte count; B, eosinophil count) were measured 48 hours after the challenge, as described in the “Methods” section. Results are shown as means ± SEM from 5 to 7 experiments. *Significantly different from mice challenged with 0.15 mol/L NaCl (P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

6 Fig. 5 LPR (at 8 hours) in passively sensitized mice challenged with DNP-OVA. Naive mice were passively sensitized by intravenous injection of anti-DNP mouse IgE (30 μg/kg) 24 hours before challenge. Mice were challenged intraperitoneally by injection with either sterile 0.15 mol/L NaCl (control) or DNP-OVA (DNP) at 30 μg/mL. Neutrophil and eosinophil numbers were measured 8 hours after the challenge, as described in the “Methods” section. Results are shown as means ± SEM from 4 experiments. *Significantly different from control (P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

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