1. Electron tomography.
Electron tomography. (A) Simian immunodeficiency virus viewed frozen hydrated and unstained in a cryo 300-kV transmission electron microscope; glycoprotein spikes and the internal core are visible. (B) Four 1-nm-thick slices from a tomogram. (C) Computer-generated three-dimensional reconstruction of one viral particle seen in panel B. Bars, 50 nm. Magnification, ×100,000. (Reprinted from reference 85.)‏
Cynthia S. Goldsmith, and Sara E. Miller Clin. Microbiol. Rev. 2009; doi: /CMR