1. Computational analysis of the Msi1 3′UTR sequence and identification of the associated RBP activities.
Computational analysis of the Msi1 3′UTR sequence and identification of the associated RBP activities. (A) Alignment of the mammalian Msi1 3′UTR sequences is shown: Homo sapiens (Hs, NM_002442) at the top, Rattus norvegicus (Rn, CD568097) in the middle and Mus musculus (Mm, NM_008629) at the bottom. Conserved nucleotides are highlighted in grey, whereas the ARE is indicated by the box. (B) The most stable RNA secondary structure of the mouse Msi1 3′UTR as predicted by the Sfold software ( The magnification shows the loop exposing the ARE sequence, indicated by the arrow. (C) UV crosslinking assay of Msi1 and Gap43 3′UTR riboprobes and lysates from whole mouse brain and NSCs. The 95 kDa complex is detected only when brain extracts were used. The 37 and 32 kDa RNA-binding activities specific for Msi1 sequence are indicated by asterisks. (D) Immunoprecipitation of Msi1 and Gap43 riboprobes with the anti-nELAV antibody after UV irradiation on brain and NSC extracts. A 42 kDa complex (arrowhead) was precipitated in all samples. (E) Immunoprecipitation of the NSC UV crosslinked mRNPs with the anti-nELAV and the anti-Msi1 antibodies, respectively.
Antonia Ratti et al. J Cell Sci 2006;119:
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