1. Exploring a Putative Gene
גיא וקסמן
רועי נבון

2. Problem definition Taking one of the thousands of
Putative genes that were “found”
In the human genome project &
Trying to determine whether this
Sequence might be a real gene that can
be translated into a protein product.

3. The Selected Gene:
NM_014727
Homo sapiens myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4).

4. PSI-BLAST

5. The Locus problem Many entrances are actually on the
same locus (SNPs, flanking, different
Alleles etc`).

6. Different Entrances on the Same Chromosomal Region

7. Conserved Domains
CXXC zinc finger
SET1
Zinc finger

8. 2-BLAST
2-BLAST between the putative MLL4 gene & the most similar real Gene-Trithorax, from Drosophila
Melanogaster.
E=5e-87
E=2e-82

9. Multiple Alignment
SET1 domain

10. 3D Structure – Zinc Finger Domain

11. Biological Aspects Drosophila melanogaster ככה נראיתי פעם...
The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains. Mazo AM, Huang DH, Mozer BA, Dawid IB
ככה נראיתי פעם...

12. Homo Sapians
Acute mixed-lineage leukemia t(4;11)(q21;q23) generates an MLL-AF4 fusion product

13. Conclusions 1. We found a high similarity between our
putative gene and genes from other
species.
2. The MLL4 putative gene contains at least 3
conserved domains.
3. The homologue genes from Drosophila and
Human are associated with the nervous
system and leukemia respectively.

14. Possible directions for future research
1. Trying to find DNA motifs which bind to our
putative gene and to its homologues.
2. Trying to isolate the full length protein.
3. When 3D structure prediction will be
available, we’ll try to compare our putative
protein with other known proteins.