1. Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles 
Giannina Calongos, M.D., Akiko Hasegawa, Ph.D., Shinji Komori, M.D., Ph.D., Koji Koyama, M.D., Ph.D. 
Fertility and Sterility 
Volume 89, Issue 5, Pages (May 2008)
DOI: /j.fertnstert
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Diameters and growth profile of preantral follicles cultured with urinary and recombinant FSH for 10 days. Data represent diameters (mean ± SD), and asterisks (∗) above bars indicate a statistically significant difference on each day of culture (P<.05, Student's t-test).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Growth and development of preantral follicles cultured with urinary and recombinant FSH. Follicles cultured with urinary (A-C) and recombinant FSH (D-F) at day 0, day 5, and day 10 are shown. Follicles from both groups show progressive increase in size and differentiation to antral follicles (original magnification ×40). Typical follicles cultured with urinary (G-I) and recombinant FSH (J-L) at day 0, day 4, and day 7 are shown. Antral formation (arrows) can be noticed with the increase in size of follicles and the proliferation of granulosa cells (∗). A typical antral follicle had significant antrum and a well-distinguished oocyte on day 7 in urinary FSH (I), but the antrum and the oocyte were not clearly seen in recombinant FSH (L) because of the mass of granulosa cells. Scale bars represent 100 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Comparison of antral formation, mucification, and mature oocyte rates between urinary and recombinant FSH. Statistically significant values are written above the columns based on the chi-square test. (N.S.: not statistically significant.)
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Comparison of embryo development to the two-cell, four-cell, morula, and blastocyst stage between urinary and recombinant FSH. Statistically significant values are written above the columns based on the chi-square test. (N.S.: not statistically significant.)
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

6. Figure 5
17β-estradiol values during in vitro growth days (A) and its relationship to numbers of cells per follicle cultured with urinary (B) or recombinant FSH (C). Asterisks (∗) above bars indicate a statistically significant difference between urinary and recombinant FSH on each day of culture (P<.001, Student's t-test). We used 14 samples for analysis of the relationship between cell number and estradiol concentration in each group. Values of each follicle are represented by dots. The tendency lines were drawn in the middle of the figure with its respective formula obtained by Microsoft Excel.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions