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Acute Pseudomonas challenge in cystic fibrosis mice causes prolonged nuclear factor- κB activation, cytokine secretion, and persistent lung inflammation 

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Presentation on theme: "Acute Pseudomonas challenge in cystic fibrosis mice causes prolonged nuclear factor- κB activation, cytokine secretion, and persistent lung inflammation "— Presentation transcript:

1 Acute Pseudomonas challenge in cystic fibrosis mice causes prolonged nuclear factor- κB activation, cytokine secretion, and persistent lung inflammation  Aicha Saadane, PhD, Jindrich Soltys, DVM, PhD, Melvin Berger, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 117, Issue 5, Pages (May 2006) DOI: /j.jaci Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Quantitative bacteriology in CF-KO (squares, n = 11) and WT (circles, n = 13) mice inoculated with P aeruginosa. Values represent the mean ± SEMs of log10 of CFU per mouse lung 2, 4, and 6 days after inoculation. The CFUs at day 0 indicate the inoculating dose. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Percent PMNs in BAL fluid in CF-KO (squares, n = 26) and WT (circles, n = 25) mice inoculated with P aeruginosa. Results shown are means ± SEMs of the percent PMNs in BAL fluid 2, 4, and 6 days after inoculation. The 0 time point is for unchallenged controls. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 BAL fluid proinflammatory cytokines in CF-KO (squares, n = 26) and WT (circles, n = 25) mice inoculated with P aeruginosa. Means ± SEMs of concentrations in BAL fluid 2, 4, and 6 days after inoculation. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 A, Representative EMSA for activated NF-κB in homogenates of perfused whole lungs from CF-KO and WT mice that had undergone BAL and were killed 2, 4, and 6 days after P aeruginosa. B, The amount of complexed NF-κB probe estimated by image scanning and expressed in arbitrary units; CF-KO, closed bars, and WT mice, open bars. Three separate experiments are included. Results shown are means ± SEMs for 3 to 4 mice for each bar. C, Controls for specificity of EMSA: lane 1, labeled probe with extract known to contain active NF-κB; lane 2, labeled probe with no extract; lane 3, labeled probe with extract plus excess of unlabeled NF-κB oligonucleotide; lane 4, labeled probe with extract plus excess of unlabeled AP-1 oligonucleotide. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 A, Western blots showing time course of I-κBα content of homogenate of whole lung from CF-KO versus WT mice inoculated with P aeruginosa. Equal protein application was verified by equal staining for β-actin. Representative of 3 separate experiments. B, Densitometric scan area of total I-κBα on Western blots corrected by normalization to scan area of β-actin. Results are average values of 3 independent experiments. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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