1. Fatty acid incorporation into triglycerides, triglyceride content, glycerol release, and fatty acid efflux in wild-type and +CD36 myotubes.
Fatty acid incorporation into triglycerides, triglyceride content, glycerol release, and fatty acid efflux in wild-type and +CD36 myotubes. A: Differentiated myotubes were incubated for 2 h with 14C-palmitate in KRH buffer (fatty acid–to–BSA ratio = 2). Label recovery into triglycerides was determined from thin-layer chromatography of lipid extracts of washed cells. Alternatively, myotubes were assayed for triglyceride content and glycerol release. For glycerol release, cells were washed and incubated for 3 h in KRH with 2% BSA and 5 mmol/l glucose. B: HSL mRNA abundance was determined by RT-PCR. Data are means ± SD from two separate experiments with triplicate determinations. C: Cells were preincubated (4 min) with 3H-palmitate, quickly washed, and incubated in KRH with 2% BSA. At the indicated times, cells were lysed in 0.1N NaOH and aliquots were counted. Cell radioactivity is expressed as percentage of initial radioactivity at the end of the preincubation. Data shown are means ± SD of triplicates from two different experiments. *P < 0.05.
Claire C. Bastie et al. Diabetes 2004;53:
©2004 by American Diabetes Association