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Figure 1 Collagen is associated with ChemR23 expression in human and murine vascular smooth muscle cells. (A) Positive ... Figure 1 Collagen is associated.

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Presentation on theme: "Figure 1 Collagen is associated with ChemR23 expression in human and murine vascular smooth muscle cells. (A) Positive ... Figure 1 Collagen is associated."— Presentation transcript:

1 Figure 1 Collagen is associated with ChemR23 expression in human and murine vascular smooth muscle cells. (A) Positive ... Figure 1 Collagen is associated with ChemR23 expression in human and murine vascular smooth muscle cells. (A) Positive association between ChemR23 and Col1A1 mRNA in human arteries (n = 60 patients; regression coefficient r = 0.538, P < 0.0001). (B) Mouse VSMC Col1A1 mRNA and protein expression after 24 h in culture. Data are represented as mean ± standard deviation of 3–5 independent experiments. *P < 0.05; **P < 0.01. Unless provided in the caption above, the following copyright applies to the content of this slide: Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) For permissions, please article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( Cardiovasc Res, cvy316, The content of this slide may be subject to copyright: please see the slide notes for details.

2 Figure 2 ChemR23 deletion alters aortic smooth muscle cell phenotype in vitro. (A) mRNA expression of genes encoding ... Figure 2 ChemR23 deletion alters aortic smooth muscle cell phenotype in vitro. (A) mRNA expression of genes encoding contractile proteins after 24 h and 9 days in culture. (B) Aortic smooth muscle cell proliferation after 72 h measured by <sup>3</sup>H-thymidine incorporation (expressed in counts per minute, cpm). (C) mRNA expression of genes relevant for calcification. Data are represented as mean ± standard deviation of 3–8 independent experiments. *P < 0.05; **P < 0.01. Unless provided in the caption above, the following copyright applies to the content of this slide: Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) For permissions, please article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( Cardiovasc Res, cvy316, The content of this slide may be subject to copyright: please see the slide notes for details.

3 Figure 3 ChemR23 deficient VSMCs exhibit reduced calcification and osteogenic signalling. (A) Quantification of ... Figure 3 ChemR23 deficient VSMCs exhibit reduced calcification and osteogenic signalling. (A) Quantification of phosphate-induced calcification after 9 days and representative images of alizarin red stained mouse aortic smooth muscle cells. (B) mRNA expression of genes involved in calcification in VSMCs after 9 days in culture under calcifying conditions. (C) Immunoblotting and immunofluorescence against phosphorylated SMAD 1/5/8 after 30 min stimulation with BMP-2 (D) mRNA expression and supernatant OPG protein levels. Quantification and representative images of phosphate-induced VSMC calcification after antibody mediated neutralization of OPG. Data are represented as mean ± standard deviation of 3–9 independent experiments. *P < 0.05; **P < 0.01. Unless provided in the caption above, the following copyright applies to the content of this slide: Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) For permissions, please article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( Cardiovasc Res, cvy316, The content of this slide may be subject to copyright: please see the slide notes for details.

4 Figure 4 ChemR23 deletion protects from VitD3 induced vascular calcification in vivo. (A) Quantification of aortic ... Figure 4 ChemR23 deletion protects from VitD3 induced vascular calcification in vivo. (A) Quantification of aortic root calcification as a percentage of the alizarin red positive area within the total vessel area, and representative images of alizarin red and Von Kossa stained aortic roots. (B) Total Ca<sup>+2</sup> content in the right carotid artery. (C) Immunoblotting of the aorta against phosphorylated SMAD 1/5/8 (D) Bone mineral density of the right femur. (E) Aortic mRNA expression of smooth muscle cell markers. (F) Col1A1 immunohistochemistry quantification, and representative photomicrographs of the aortic root. (G) Aortic mRNA expression of genes involved in calcification promotion and inhibition. (H) Plasma OPG levels. (I) Plasma phosphate levels. Data are represented as mean ± standard deviation. ChemR23<sup>+/+</sup>n = 5, ChemR23<sup>−/−</sup>n = 6. *P < 0.05; **P < 0.01. Unless provided in the caption above, the following copyright applies to the content of this slide: Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) For permissions, please article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( Cardiovasc Res, cvy316, The content of this slide may be subject to copyright: please see the slide notes for details.

5 Figure 5 Effects of resolvinE1 (RvE1) and (A) quantification of phosphate-induced calcification after 9 days and ... Figure 5 Effects of resolvinE1 (RvE1) and (A) quantification of phosphate-induced calcification after 9 days and representative images of alizarin red stained cells treated with either vehicle (Ethanol) or RvE1. (B) mRNA expression of VSMCs treated with RvE1 or vehicle for 9 days under calcifying conditions. Data are represented as mean ± standard deviation. *P < 0.05; **P < 0.01. Unless provided in the caption above, the following copyright applies to the content of this slide: Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) For permissions, please article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( Cardiovasc Res, cvy316, The content of this slide may be subject to copyright: please see the slide notes for details.

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