1. Abnormally low or high Tj expression causes a decrease in DEcad expression and shg enhancer activity.
Abnormally low or high Tj expression causes a decrease in DEcad expression and shg enhancer activity. (A-F) Arrows point to BCCs (magnified in A′-F′). (A-C′) Contrary to the control (A), DEcad does not appear enriched in the BCC when Tj is reduced (tj RNAi) (B) and appears very weak when Tj is overexpressed (OE) (C). (D-F′) Similarly, contrary to the control, shg-lacZ signal is weak when Tj is reduced (E) or overexpressed (F). (G-H′) Mosaic BCCs (white arrows, magnified in G′-H′). Tj-overexpressing rosette cells (yellow arrows), identified by β-gal (G′,H′), display significantly lower DEcad expression compared with their wild-type neighbors (G′,H′), and are located at the trailing edge of the BCC at stages 9 (G) and 10 (H). Scale bars: 20 μm in A-H; 10 μm in A′-H′.
Felix Gunawan et al. Development 2013;140:
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