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Luis Condezo-Hoyos, PhD, María Rubio, MD, Silvia M

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Presentation on theme: "Luis Condezo-Hoyos, PhD, María Rubio, MD, Silvia M"— Presentation transcript:

1 A plasma oxidative stress global index in early stages of chronic venous insufficiency 
Luis Condezo-Hoyos, PhD, María Rubio, MD, Silvia M. Arribas, PhD,, Gabriel España-Caparrós, PhD, Pilar Rodríguez-Rodríguez, MSc, Elio Mujica-Pacheco, PhD, M. Carmen González, PhD,  Journal of Vascular Surgery  Volume 57, Issue 1, Pages (January 2013) DOI: /j.jvs Copyright © 2013 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Plasma antioxidant capacity. A, Fluorescence decay curves induced by the peroxyl radical generator 2,2′-azobis-2-methyl-propanimidamide dihydrochloride in the absence (white circles) and the presence (black triangles) of plasma from patients with chronic venous insufficiency and healthy controls. The inset shows oxygen radical antioxidant capacity (ORAC) values. B, Variation in the luminescence signal produced in Luminol-HRP-Hydrogen peroxide system before and after addition of plasma from patients with chronic venous insufficiency or healthy controls. The inset figure shows total antioxidant capacity values. Data represent the mean ± standard error of the mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Plasma antioxidant defense systems: (A) total thiols content, (B) reduced glutathione, (C) uric acid, and (D) catalase activity in patients with chronic venous insufficiency and healthy controls. Data represent the mean ± standard error of the mean. **P < .001 vs control subjects. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

4 Fig 3 Parameters related to protein oxidative damage: (A) protein-bound malondialdehyde (MDA), (B) protein carbonyls, (C) 3-nitrotyrosine (3-NT), and (D) advanced oxidation products measured in plasma from patients with chronic venous insufficiency and healthy controls. Data represent the mean ± standard error of the mean. **P < .001 with respect to control. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Activity of enzymes related to reactive oxygen species (ROS) production: (A) myeloperoxidase (MPO) and (B) xanthine oxidase activities in plasma from patients with chronic venous insufficiency and healthy controls. Data represent the mean ± standard error of the mean. MPO, Myeloperoxidase. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

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