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Fig. 5. Upregulation of N. vectensis thrombospondin during regeneration.(A,B) Juvenile polyps were fixed 48 hours following transection and processed for whole mount in situ hybridization with an antisense (A) or sense (B) probe to Nv Upregulation of N. vectensis thrombospondin during regeneration.(A,B) Juvenile polyps were fixed 48 hours following transection and processed for whole mount in situ hybridization with an antisense (A) or sense (B) probe to Nv There is a superficial signal in the column wall of the polyp that appears stronger near the site of transection (arrows). Scale bars = 500 µm. (C) Quantitative PCR reveals a 41.8±11.1 (n = 3) fold increase in Nv transcripts at 48 hours after transection. The fold increase was normalized to the levels of expression of an rRNA control transcript. (D,E) Longitudinal sections through the body wall of an adult N. vectensis polyp at 48 hours after transection were immunostained with anti-NvTSP The antibody labels the glycocalyx lining the epidermis (arrows) in addition to the neuron-like elements in the retractor muscles (rm). The immunostaining of the glycocalyx ends near the oral end of the column (E). gv, gastrovascular cavity; pm, parietal muscle. Scale bars = 25 µm (D,E). Richard P. Tucker et al. Biology Open 2013;2: © Published by The Company of Biologists Ltd
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