1. Fig. 1. MSCs undergo in vivo apoptosis after infusion without affecting immunosuppression.
MSCs undergo in vivo apoptosis after infusion without affecting immunosuppression. (A) Luc-MSCs were injected intravenously into naïve, BM, and GvHD mice 3 days after transplantation. All animals were then injected intraperitoneally with Z-DEVD-aminoluciferin and imaged 1 hour later. n = 6 (one to three mice per group), grouped from three independent experiments. In each experiment, a different MSC expansion was used. White lines separate multiple photographs assembled in the final image. (B) TLS was measured from the images of mice in (A) and shown as mean ± SD. (C and D) Percentage of GvHD effector cells (CD8+Vβ8.3+) calculated in the lymphocyte gate (defined by the physical characteristics of the cells) in the spleen (C) and lungs (D) of GvHD mice (black circles) and GvHD mice treated with MSCs (black squares) 4 days after MSC injection. n = 15 (GvHD) and 13 (GvHD + MSCs) mice, grouped from four independent experiments. Means ± SD are shown. Statistics in (B): one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test (**P < 0.01 and ***P < 0.001). Statistics in (C) and (D): unpaired t test (**P < 0.01). ns, not significant.
Antonio Galleu et al., Sci Transl Med 2017;9:eaam7828
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