1. APCs from hypertensive mice present antigens more efficiently.
APCs from hypertensive mice present antigens more efficiently. (A and B) Splenic DCs and peritoneal macrophages (PM) from normotensive or Ang II–induced hypertensive C57BL/6 mice were pulsed with 10 μM OVA (A) or 50 pM SIINFEKL peptide (SKL) (B) in vitro and were then coincubated with OT-I T cells. The percentages of CD69+ (after 4 hours) or IFN-γ+ cells (after 6 hours in the presence of brefeldin A) among OT-I T cells were measured by flow cytometry. Representative flow cytometry dot plots of IFN-γ and CD69 expression are shown. (C) Splenic DCs from normotensive or L-NAME–induced hypertensive mice were pulsed with SIINFEKL and then coincubated with OT-I T cells. The percentages of CD69+ or IFN-γ+ cells among all CD8+ T cells were measured. (D) Splenic DCs from normotensive or Ang II–induced hypertensive mice were loaded with SIINFEKL for 4 hours ex vivo. Cells were then transferred intravenously into naïve C57BL/6 mice. Seven days later, splenocytes from the recipients were restimulated with SIINFEKL and the secretion of IL-2 and IFN-γ was measured. Data are means ± SEM. *P < 0.05; **P < 0.01; ***P <
Tuantuan V. Zhao et al. Sci. Immunol. 2019;4:eaau6426
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