Presentation is loading. Please wait.

Presentation is loading. Please wait.

Prostaglandin E receptor subtype EP3 in conjunctival epithelium regulates late-phase reaction of experimental allergic conjunctivitis  Mayumi Ueta, MD,

Published byLennart Dahlberg Modified over 5 years ago

Similar presentations

Presentation on theme: "Prostaglandin E receptor subtype EP3 in conjunctival epithelium regulates late-phase reaction of experimental allergic conjunctivitis  Mayumi Ueta, MD,"— Presentation transcript:

1 Prostaglandin E receptor subtype EP3 in conjunctival epithelium regulates late-phase reaction of experimental allergic conjunctivitis  Mayumi Ueta, MD, PhD, Toshiyuki Matsuoka, MD, PhD, Shuh Narumiya, MD, PhD, Shigeru Kinoshita, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 123, Issue 2, Pages e5 (February 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Expression and localization of EP3 in the conjunctiva and cornea. A, RT-PCR analyses of the expression of EP3-specific mRNA in the cornea and conjunctiva of wild-type mice. B, Histochemical staining for EP3 (X-gal): wild-type mice (a) and Ptger3−/− mice (b-d). Data are representative of 3 experiments. C, Immunohistologic analysis for EP3. Each bar represents a length of 500 (B, a, b), 50 (B, c, d), or 100 (C) μm. Data are representative of 3 experiments. ∗Conjunctival epithelium, #corneal epithelium, ∋Corneal endothelium. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Histologic analyses of eosinophil inflammation in conjunctiva. Infiltration of eosinophils into the conjunctiva of wild-type and Ptger3−/− mice were detected by using the Luna method, which stained eosinophil granules with a distinctive red. Scale bars = 50 μm. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Real-time PCR analyses of the expression of eotaxin-1 mRNA in the eyelids 6 hours after RW challenge. Relative mRNA expression levels for eotaxin-1 normalized by GAPDH are shown. Data are shown as the mean ± SEM of 7 samples per group. ∗P < .05. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 RW challenge increased PGE2 synthesis in eyelids. A, Time course of COX-2 and mPGES-1 mRNA expression in eyelids during EAC. The y-axis shows the increase of specific mRNA over that seen in 0-hour samples. The x-axis shows the time after the RW challenge. Data are shown as the mean ± SEM of 3 samples. B, Time course of PGE2 content in eyelids during EAC. The x-axis shows the time after the RW challenge. Data are shown as the mean ± SEM of 4 samples. C, Localization of mPGES-1 on the ocular surface. Each bar represents a length of 100 μm. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 The effects of an EP3-selective agonist on eosinophil infiltration in conjunctiva. Data are shown as the mean ± SEM of samples from all the mice examined. Negative control (RW sensitization without challenge), positive control (RW sensitization with challenge) only vehicle, and AE248 are shown. (Wild-type mice: negative control, n = 20; positive control, n = 20; vehicle only, n = 25; AE248, n = 30; Ptger3−/− mice: negative control, n = 11; positive control, n = 11; vehicle only, n = 14; AE248, n = 16). ∗∗∗∗∗P < Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Antibody responses to RW sensitization and challenge
Antibody responses to RW sensitization and challenge. The serum concentration of total IgE (left) and the log titer of RW-specific IgE (middle) and RW-specific IgG1 (right) assessed by means of ELISA are shown. Sera were prepared from sensitized wild-type and Ptger3−/− mice at 24 hours after RW challenge. Sera from unsensitized wild-type mice were used as controls. Data are shown as the means ± SEMs of 8 samples. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 The optimal dose of ONO-AE-248
The optimal dose of ONO-AE-248. ONO-AE-248 was administered at various doses (0.01 ng, 0.1, 1, 10, 100 ng) in 5 μL of PBS per eye to wild-type mice at 1, 3, and 7 hours after RW challenge. The quantified eosinophil numbers in conjunctivas of wild-type mice were shown. The data from the mice without both RW challenge and topical treatment and the mice without topical treatment were used as negative and positive controls, respectively. Data are shown as the means ± SEMs of samples from all mice examined. Positive control (RW sensitization with challenge), n = 19; negative control (RW sensitization without challenge), n = 19. AE248: 0.01 ng, n = 18; 0.1 ng, n = 6; 1 ng, n = 27; 10 ng, n = 6; 100 ng, n = 17. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Infiltration of eosinophils into the conjunctiva treated with an EP3-selective agonist. Infiltrations of eosinophils were detected by using the Luna method, which stained eosinophil granules with a distinctive red. Scale bars = 50 μm. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 The expression of EP3 in human conjunctival epithelium
The expression of EP3 in human conjunctival epithelium. A, RT-PCR analyses of the expression of EP3-specific mRNA. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. B, Immunohistologic analysis for EP3. Each bar represents a length of 50 μm. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Prostaglandin E receptor subtype EP3 in conjunctival epithelium regulates late-phase reaction of experimental allergic conjunctivitis  Mayumi Ueta, MD,"

Similar presentations

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity  Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,

Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity  Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,
Loss of sirtuin 1 (SIRT1) disrupts skin barrier integrity and sensitizes mice to epicutaneous allergen challenge  Mei Ming, PhD, Baozhong Zhao, PhD, Christopher.

Loss of sirtuin 1 (SIRT1) disrupts skin barrier integrity and sensitizes mice to epicutaneous allergen challenge  Mei Ming, PhD, Baozhong Zhao, PhD, Christopher.
Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma  Kyung S. Lee, MS, Sun M. Jin,

Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma  Kyung S. Lee, MS, Sun M. Jin,
Platelets play important roles in the late phase of the immediate hypersensitivity reaction  Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo.

Platelets play important roles in the late phase of the immediate hypersensitivity reaction  Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo.
Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M
Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,

Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,
Epistatic interaction between Toll-like receptor 3 (TLR3) and prostaglandin E receptor 3 (PTGER3) genes  Mayumi Ueta, MD, PhD, Gen Tamiya, PhD, Katsushi.

Epistatic interaction between Toll-like receptor 3 (TLR3) and prostaglandin E receptor 3 (PTGER3) genes  Mayumi Ueta, MD, PhD, Gen Tamiya, PhD, Katsushi.
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Clemens Feistritzer, MD, Birgit A. Mosheimer, MD, Daniel H

Clemens Feistritzer, MD, Birgit A. Mosheimer, MD, Daniel H
A novel allergen-specific therapy for allergy using CD40-silenced dendritic cells  Motohiko Suzuki, MD, PhD, Xiufen Zheng, PhD, Xusheng Zhang, MD, PhD,

A novel allergen-specific therapy for allergy using CD40-silenced dendritic cells  Motohiko Suzuki, MD, PhD, Xiufen Zheng, PhD, Xusheng Zhang, MD, PhD,
Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,
Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J

Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J
FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling  Jennifer Kearley, PhD, Douglas S. Robinson,

CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling  Jennifer Kearley, PhD, Douglas S. Robinson,
Cheng-Chiu Huang, PhD, Yu Shin Kim, PhD, William P

Cheng-Chiu Huang, PhD, Yu Shin Kim, PhD, William P

Similar presentations

Ads by Google