1. Interleukin-1 blockade effects interferon (IFN)-γ production by multiple cell types. a) Gating strategy for flow cytometry: CD56+ natural killer (NK) cells (CD3−CD56+), CD3+ T-cells (CD3+CD56−) and other cells (CD3−CD56−) were identified within the total ga...
Interleukin-1 blockade effects interferon (IFN)-γ production by multiple cell types. a) Gating strategy for flow cytometry: CD56+ natural killer (NK) cells (CD3−CD56+), CD3+ T-cells (CD3+CD56−) and other cells (CD3−CD56−) were identified within the total gated lymphocytes. The percentage of IFN-γ-producing cells was then evaluated in each of the cell subtypes. b) Percentage of non-typeable Haemophilus influenzae (NTHi)-stimulated IFN-γ-producing cells in the absence and presence of interleukin-1 receptor antagonist (IL-1Ra) at 24 h post-stimulation (n=9). *: p<0.05 by Wilcoxon matched-pairs signed rank test; **: p<0.01 by Wilcoxon matched-pairs signed rank test.
Alice C-H. Chen et al. ERJ Open Res 2017;3:
©2017 by European Respiratory Society