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Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion.

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Presentation on theme: "Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion."— Presentation transcript:

1 Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion fibroblasts  Natalie N. Rizk, Ph.D., Ghassan M. Saed, Ph.D., Michael P. Diamond, M.D.  Fertility and Sterility  Volume 86, Issue 4, Pages (October 2006) DOI: /j.fertnstert Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 Real-time PCR of the insulin receptor after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts, (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Sample size, three patients (mean ± SEM; *P<.05 compared with column A, #P<.05 compared with column A′; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 Real-time PCR of the IGF-1 receptor after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts, (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Sample size, three patients (mean ± SEM; *P<.05 compared with column A, #P<.05 compared with column A′; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 Real-time PCR of IGF-1 after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts, (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Sample size, three patients (mean ± SEM; *P<.05 compared with column A, #P<.05 compared with column A′; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5 FIGURE 4 Concentrations of IGF-1 (in pg/mL) after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Concentrations of IGF-I were measured in medium in which cells were incubated. Sample size, three patients (mean ± SEM; *P<.05 compared with normal fibroblasts at 100 mg/dL; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions


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