1. SHU2 mutants have phenotypes similar to the Rad51 paralogs.
SHU2 mutants have phenotypes similar to the Rad51 paralogs. (A) Disruption of SHU1 or SHU2 leads to decreased rates of Rad51-dependent repair. Diagram of direct repeat recombination assay (leu2-∆EcoRI::URA3::leu2-∆BstEII) used to measure rates of direct-repeat recombination by Rad51-dependent GC (right side of diagram, Ura+Leu+ colonies) or Rad51-independent SSA (left side of diagram, Ura−Leu+ colonies). The rates of GC and SSA events in shu1∆ or shu2∆ cells were compared with WT and standard deviations shown. (B) Shu1 bridges the interaction between Shu2 and Psy3. Yeast two-hybrid analysis of pGBK-SHU2 (containing a GAL4-binding domain) with pGAD-SHU1, pGAD-CSM2, pGAD-PSY3, and pGAD-C1 empty plasmid (containing a GAL4-activating domain) in the presence or absence of the endogenous SHU1 gene. Interaction is indicated by growth on medium lacking histidine. Growth on medium lacking leucine and tryptophan selects for the individual plasmids and serves as a loading control.
Stephen K. Godin et al. Genetics 2015;199:
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