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De novo generation of cervid prions.
De novo generation of cervid prions. (A) Representative Western blots of de novo prions generated by sPMCA. Samples in all lanes were digested with 50 µg/ml proteinase K except lane 1, and all lanes contain NBH amplified by PMCA except lane 12, which contains unamplified deer prions for comparison. De novo prions are indicated by an asterisk (*). (B) Rate of de novo-generated prions at the respective rounds. Gray bars indicate the rate of de novo generation for that individual round, and black bars indicate the cumulative rate. Crystal Meyerett-Reid et al. mSphere 2017; doi: /mSphere
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