1. Functional analysis of Mapk14 in SSCs by spermatogonial transplantation.
Functional analysis of Mapk14 in SSCs by spermatogonial transplantation. (A) Macroscopic appearance of a recipient testis that underwent transplantation of AxCANCre-treated Mapk14f/f testis cells. Scale bar: 1 mm. (B) Colony counts (n = 25 testes for Mapk14f/f; n = 20 testes for control). *P < 0.05 (t test). (C) Histological appearance of a recipient testis. Scale bar: 250 μm. (D) Quantification of tubules exhibiting spermatogenesis. At least 1,139 tubules were counted. *P < 0.05 (t test). (E) Defective proliferation of Mapk14f/f GS cells exposed to AxCANCre (n = 14 cultures; moi = 2). The cells were recovered 6 d after transfection. *P < 0.05 (t test). (F) Flow cytometric analysis of ROS generation in AxCANCre-treated Mapk14f/f GS cells 1 d after transfection (moi = 2; n = 3 cultures). *P < 0.05 (linear regression). (G) Real-time PCR analysis of Nox1 and Noxa2 expression in Mapk14f/f GS cells exposed to AxCANCre 4 d after infection (n = 3 cultures; moi = 2). *P < 0.05 (t test). Data information: in (B, D–G), data are presented as mean ± SEM.
Hiroko Morimoto et al. LSA 2019;2:e
© 2019 Morimoto et al.