1. Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens 
Juan A. Asturias, PhD, Ignacio Ibarrola, PhD, Angel Ferrer, MD, PhD, Carmen Andreu, MD, Esperanza López-Pascual, MD, Joaquín Quiralte, MD, PhD, Fernando Florido, MD, PhD, Alberto Martínez, PhD 
Journal of Allergy and Clinical Immunology 
Volume 115, Issue 6, Pages (June 2005)
DOI: /j.jaci
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2. Fig 1
A, Coomassie-stained SDS-PAGE and immunoblots of A alternata allergens. nAlt a 1 (lanes 1), rAlt a 2 (lanes 2), and rAlt a 11 (lanes 3) were run in the presence and absence of β-mercaptoethanol (βME). Immunoblots were incubated with a human serum pool of patients with A alternata allergy. B, Dot blots for nAlt a 1, rAlt a 2, rAlt a 11, and BSA spotted onto nitrocellulose membrane. Dot blots were incubated with individual sera from patients with A alternate allergy.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3. Fig 2
A, Coomassie-stained SDS-PAGE and immunoblot of A alternata allergens. nAlt a 1 (lanes 1), rbAlt a 1 (lanes 2), and ryAlt a 1 (lanes 3) were run in the presence and absence β-mercaptoethanol (βME). Immunoblots were incubated with a human serum pool of patients with A alternata allergy. B, EAST inhibition curves. The binding of human IgE to A alternata extract was inhibited with the following: A alternata extract, nAlt a 1, rbAlt a 1, and ryAlt a 1. Values represent means ± SD.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4. Fig 3
SPT with A alternata extract (AAE), and 3 different concentrations of purified natural and rAlt a 1 are shown. Values are given as means of wheal surface areas. ∗Difference significant at P < .05. NS, Not significant.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions