1. Changes in DNA fragmentation during sperm preparation for intracytoplasmic sperm injection over time 
Natalia Rougier, M.D., Heydy Uriondo, M.Sc., Sergio Papier, M.D., Miguel Angel Checa, M.D., Ph.D., Carlos Sueldo, M.D., Cristian Alvarez Sedó, M.Sc. 
Fertility and Sterility 
Volume 100, Issue 1, Pages (July 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Terminal deoxynucleotide transferase–mediated dUTP nick-end labeling (TUNEL) assay. The negative control was performed by omitting the “enzyme” solution [phase contrast (A′), under green fluorescence (A), and dark field + fluorescence]. A DNAse-treated sample was used as the positive control [green fluorescence, 100% of positive cells (B), phase contrast (B′), and dark field + fluorescence (B″)]. Only complete spermatozoa (C, arrow) were considered in the evaluation of TUNEL; sperm heads without tails were not counted for this purpose (C, arrowhead).
Fertility and Sterility  , 69-74DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
DNA fragmentation dynamics. The levels of DNA fragmentation decreased significantly after obtaining a centrifugation gradient in all samples (*). In patients with terminal deoxynucleotide transferase–mediated dUTP nick-end labeling (TUNEL) <20% (A), we observed a significant increase of DNA fragmentation after TG2-90 (***) (10.2 ± 3% to 13.4 ± 4%). In patients with TUNEL ≥20% (B), we observed a significant increase in DNA fragmentation beginning at TG2-60 (**) (25.3 ± 5 to 30.4 ± 6). HA = hyaluronic acid; PVP = polyvinylpyrrolidone.
Fertility and Sterility  , 69-74DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Sperm DNA fragmentation dynamics at various time points. T0 (A), TG (B), TG1 (C), TG2 (D), TG2-30 (E), TG2-60 (F), and TG2-90 in polyvinylpyrrolidone (G) and TG2–30 (E′), TG2–60 (F′), and TG2–90 (G′) in hyaluronic acid. Images of green fluorescence/dark field and phase contrast microscopy (insets) were captured.
Fertility and Sterility  , 69-74DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions