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Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue  Ying-Hung Lin, M.Sc., Yung-Ming Lin,

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Presentation on theme: "Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue  Ying-Hung Lin, M.Sc., Yung-Ming Lin,"— Presentation transcript:

1 Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue  Ying-Hung Lin, M.Sc., Yung-Ming Lin, M.D., Yen-Ni Teng, Ph.D., Tsui-Yu Tracy Hsieh, Ph.D., Yi-Shing Lin, Ph.D., Pao-Lin Kuo, M.D.  Fertility and Sterility  Volume 86, Issue 6, Pages (December 2006) DOI: /j.fertnstert Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 Semiquantitative RT-PCR analysis for five novel genes that are down-regulated in Sertoli cell-only syndrome (SCOS). Left: Gel electrophoresis of PCR products for two testicular samples of normal spermatogenesis. Right: Gel electrophoresis of PCR products for two testicular samples from men with SCOS. A housekeeping gene (GAPDH) was used as control. 26, 29, 32, and 35 indicate cycle number of PCR. Lin. Microarray analysis of human testes. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 Semiquantitative RT-PCR analysis for eight novel genes that are down-regulated in maturation arrest (MA). Left: Gel electrophoresis of PCR products for two testicular samples of normal spermatogenesis. Right: Gel electrophoresis of PCR products for two testicular samples from men with MA. A housekeeping gene (GAPDH) was used controls. 26, 29, 32, and 35 indicate cycle number of PCR. Lin. Microarray analysis of human testes. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 Genomic structure, functional domains, size of human transcript, total number of amino acids, and chromosomal localization of novel sterile genes. The mouse orthologues were not identified for two genes (Hs and Hs ). Lin. Microarray analysis of human testes. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5 FIGURE 4 The RT-PCR analysis for expression of novel sterile genes in different human (A) and mouse (B) organs. Expression patterns of all novel sterile genes are testis-enriched in humans and mice. Expression patterns of mouse orthologues were comparable to those of human genes. Lin. Microarray analysis of human testes. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

6 FIGURE 5 Developmentally regulated expression of mouse orthologues: 1, 5, 10, 15, 20, and 35 indicate testes obtained from mice of the corresponding postnatal day. Adult mouse refers to a mouse on postnatal day 80. Postnatal days 5–10, days 15–20, and day 35, and adults correspond to mitotic phase or first meiotic division of spermatogonia, pachytene spermatocytes, postmeiotic phase, and spermiogenesis, respectively. Lin. Microarray analysis of human testes. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions


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