1. Expression of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP in serosal tissue of intraperitoneal organs and adhesions 
Nasser Chegini, Ph.D., Kristina Kotseos, B.Sc., Yong Zhao, M.D., Chunfeng Ma, M.D., Ph.D., Frederick McLean, M.D., Michael P. Diamond, M.D., Lena Holmdahl, M.D., Ph.D., James Burns, Ph.D. 
Fertility and Sterility 
Volume 76, Issue 6, Pages (December 2001)
DOI: /S (01)

2. FIGURE 1
Competitive quantitative reverse transcriptase polymerase chain reaction (PCR) analysis of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP-1 (TIMP-1) messenger RNA using total cellular RNA isolated from parietal peritoneum. The upper bands are the PCR products generated from the specific message in cellular RNA, and the lower bands are from the standard complementary RNA (shown from right to left at dilutions corresponding to 108 to 103 and 107 to 102 copies/reaction). M = DNA markers.
Chegini. MMP-1 and TIMP-1 in serosal tissue. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

3. FIGURE 2
Expression of matrix metalloproteinase (A) and tissue inhibitor of MMP-1 (B) messenger RNA (mRNA) expression in serosal tissue of parietal peritoneum, various peritoneal organs, adhesions, and skin. Values were calculated from the band densities described in Materials and Methods. Results are means ± SE of copies of mRNA/μg total RNA. In panel A, P<.05 for b compared with a, c, and d; P=.01 for b compared with g, h, j, and k; and P<.01 for a, b, c, d, e, and f compared with g, h, j, and k. In panel B, P<.05 for d compared with a, b, c, f, g, j, and k.
Chegini. MMP-1 and TIMP-1 in serosal tissue. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

4. FIGURE 3
Notched box plots showing the level of matrix metalloproteinase (MMP-1) (A) and tissue inhibitor of MMP-1 (TIMP-1) (B) in samples of serosal tissue of the ovaries, uterus, fallopian tubes (F. tube), large bowel, omentum, skin, fascia, and adhesions. The vertical line within the box boundaries represent the distribution of the middle 50%, the thin horizontal lines within the boxes are the medians, and the thick lines are the arithmetric means. The notches represent the 95th percentile, and the error bars represent the 90th and 10th percentiles. Levels of MMP-1 levels in skin, fascia, peritoneum and omentum were significantly lower than those in uterus, ovary, fallopian tube, and large bowel (P=.005, .003, and .01, respectively), and TIMP-1 levels in skin and fascia were significantly lower than those in uterus, ovary, fallopian tube, and large bowel (P=.002, .05 and .0004, respectively).
Chegini. MMP-1 and TIMP-1 in serosal tissue. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

5. FIGURE 4
Mean ± SE matrix metalloproteinase (MMP-1) (A) and tissue inhibitor of MMP-1 (TIMP-1) (B) protein content in adhesions, intact parietal peritoneum, and skin of 10 patients with adhesions. The TIMP-1 protein content in adhesions is significantly higher than that in intact parietal peritoneum (P=.05) and skin (P=.03).
Chegini. MMP-1 and TIMP-1 in serosal tissue. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

6. FIGURE 5
(A), Ratio of matrix metalloproteinase (MMP-1) to tissue inhibitor of MMP-1 (TIMP-1) in serosal tissue of the parietal peritoneum, uterus, ovaries, fallopian tubes (F. tube), large bowels, skin, fascia, omentum, and adhesions. The ratio of MMP-1 to TIMP-1 in skin, fascia, and peritoneum is significantly lower than in other tissues (P<.001) and is lower in adhesions than in uterus and ovary (P=.03 and .001, respectively). (B), Percentiles of MMP-1 associated with TIMP-1 in the tissue extracts, calculated by using the equation indicated on the Y axis. (C), Ratio of MMP-1 to TIMP-1 in adhesions, parietal peritoneum, and skin from 10 patients with adhesions. The ratio of these substances in adhesions is significantly lower than that in peritoneum and skin (P<.05 for ∗ compared with ∗∗).
Chegini. MMP-1 and TIMP-1 in serosal tissue. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )