1. Optimal Proliferation of a Hematopoietic Progenitor Cell Line Requires Either Costimulation With Stem Cell Factor or Increase of Receptor Expression That Can Be Replaced by Overexpression of Bcl-2
by Huei-Mei Huang, Jian-Chiuan Li, Yueh-Chun Hsieh, Hsin-Fang Yang-Yen, and Jeffrey Jong-Young Yen
Blood
Volume 93(8):
April 15, 1999
©1999 by American Society of Hematology

2. Proliferation synergism of SCF with IL-5 in TF-1 cells.
Proliferation synergism of SCF with IL-5 in TF-1 cells. Rapid growing TF-1 cells were washed and cultured separately in cytokine-free medium (□), SCF (50 ng/mL, ▪), GM-CSF (2 ng/mL, ○), GM-CSF+SCF (•), IL-5 (5 ng/mL, ▵), or IL-5 + SCF (▴)-containing medium. The viable cell numbers were determined daily by trypan blue staining and are shown as the percentage of the initial viable cell number. Each number represents the average from four independent duplicated experiments. The standard deviations are indicated as error bars.
Huei-Mei Huang et al. Blood 1999;93:
©1999 by American Society of Hematology

3. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

4. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

5. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

6. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

7. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

8. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

9. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

10. Overexpression of IL5R does not increase the mitogenic activity of IL-5.
Overexpression of IL5R does not increase the mitogenic activity of IL-5. Rapid growing TF-1, JYTF-1, TF1, and TF8 cells were cultured in IL-5–containing (▧) or cytokine-free (□) media for 24 hours and then 1 × 104 viable cells were subjected to [3H]thymidine pulse-labeling assay as described in Materials and Methods. For each sample, data shown is the average of 13 to 17 independent measurements (n).
Huei-Mei Huang et al. Blood 1999;93:
©1999 by American Society of Hematology

11. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

12. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

13. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

14. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

15. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology

16. Huei-Mei Huang et al. Blood 1999;93:2569-2577
©1999 by American Society of Hematology