Presentation is loading. Please wait.

Presentation is loading. Please wait.

Physiologic assessment of allergic rhinitis in mice: Role of the high-affinity IgE receptor (FcɛRI)  Satoko Miyahara, MD, PhD, Nobuaki Miyahara, MD, PhD,

Published byΧαρά Αργυριάδης Modified over 5 years ago

Similar presentations

Presentation on theme: "Physiologic assessment of allergic rhinitis in mice: Role of the high-affinity IgE receptor (FcɛRI)  Satoko Miyahara, MD, PhD, Nobuaki Miyahara, MD, PhD,"— Presentation transcript:

1 Physiologic assessment of allergic rhinitis in mice: Role of the high-affinity IgE receptor (FcɛRI) 
Satoko Miyahara, MD, PhD, Nobuaki Miyahara, MD, PhD, Katsuyuki Takeda, MD, PhD, Anthony Joetham, BS, Erwin W. Gelfand, MD  Journal of Allergy and Clinical Immunology  Volume 116, Issue 5, Pages (November 2005) DOI: /j.jaci Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 RNA 24 hours after 6 nasal challenges. RNA was assessed 24 hours after the last nasal challenge in untreated mice (PBS/PBS), nonsensitized but challenged mice (PBS/OVA6), and sensitized and challenged mice (OVA/OVA6). Nasal resistance was measured as described in the Methods section (n = 8 in each group). Means ± SEM are shown. ∗P < .01 compared with PBS/PBS or PBS/OVA6 groups. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Changes in respiratory parameters after 3 or 6 OVA challenges. RF (A), expiratory time (B), and inspiratory time (C) were monitored 24 hours after 3 or 6 nasal challenges, as described in the Methods section. Respiratory parameters were assessed in untreated mice (PBS/PBS), nonsensitized but challenged mice (PBS/OVA), and OVA-sensitized and OVA-challenged mice (OVA/OVA; n = 8 in each group). Means ± SEM are shown. ∗P < .01 compared with PBS/PBS or PBS/OVA groups. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Comparison of RNA and RF. After 4 to 6 challenges, individual RNA and RF values were compared. RNA and RF were assessed in untreated mice (PBS/PBS) and in sensitized mice after 4, 5, or 6 challenges. The correlation analysis was carried out in untreated mice (n = 4) and sensitized mice and mice challenged 4, 5, or 6 times (n = 4-8). RNA negatively correlated with RF. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 RF 24 hours after repeated allergen challenges. Twenty-four hours after the last of 4 to 12 challenges, RF was monitored in previously sensitized mice (n = 8 in each group). Means ± SEM are shown. ∗P < .05 compared with baseline values. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 A, RNA was monitored over 10 minutes after a fourth OVA challenge in sensitized (OVA/OVA3) or nonsensitized (PBS/OVA3) mice. B, RF was monitored over 60 minutes after a fourth allergen challenge in WT or FcɛRI−/− mice (n = 8 in each group). Means ± SEM are shown for RNA and RF as a percentage of the value at 2 minutes and baseline values before challenge. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001 compared with PBS/OVA3 or PBS/PBS3 groups. #P < .05, ##P < .01, and ###P < .001 compared with OVA/OVA3 FcɛRI−/− mice. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

7 Fig 6 Tissue infiltration with major basic protein–positive eosinophils 24 hours after 6 nasal challenges in WT and FcɛRI−/− mice. Immunohistochemical (major basic protein) localization of nasal septal tissue eosinophils was determined 24 hours after 6 nasal challenges in sensitized or nonsensitized mice, as described in the Methods section. Eosinophil infiltration in nonsensitized but challenged WT mice (A), sensitized and challenged WT mice (B), nonsensitized but challenged FcɛRI−/− mice (C), and sensitized and challenged FcɛRI−/− mice (D) is shown. Bar = 100 μm. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

8 Fig 7 Nasal resistance and RF 24 hours after repeated allergen challenge in FcɛRI−/− mice. RNA (A) and RF (B) were measured 24 hours after the sixth allergen challenge in nonsensitized (PBS/OVA6) and sensitized (OVA/OVA6) WT and FcɛRI−/− mice (n = 8 in each group). Means ± SEM are shown. #P < .05 and ##P < .01 compared with OVA/OVA FcɛRI−/− mice. ∗P < .05 and ∗∗P < .01 compared with PBS/OVA group. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

9 Fig 8 Levels of IL-13 in nasal tissue after sensitization and challenge. Levels of IL-13 in nasal tissue homogenates were determined 24 hours after the sixth nasal challenge, as described in the Methods section (n = 8 in each group). Means ± SEM are shown. ∗P < .01 compared with OVA/OVA FcɛRI−/− mice. #P < .01 compared with PBS/OVA6 and PBS/PBS6 groups. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Download ppt "Physiologic assessment of allergic rhinitis in mice: Role of the high-affinity IgE receptor (FcɛRI)  Satoko Miyahara, MD, PhD, Nobuaki Miyahara, MD, PhD,"

Similar presentations

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Allergen immunotherapy: A practice parameter third update

Allergen immunotherapy: A practice parameter third update
Pharmacologic rationale for treating allergic and nonallergic rhinitis

Pharmacologic rationale for treating allergic and nonallergic rhinitis
High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Ann-Marie M. Schoos, MD, PhD, Jacob D

Ann-Marie M. Schoos, MD, PhD, Jacob D
Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,

Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,
Major rabbit allergen Ory c 3: What could be its possible role as a sensitizing agent in real life?  Gennaro Liccardi, MD, Antonello Salzillo, MD, Maria.

Major rabbit allergen Ory c 3: What could be its possible role as a sensitizing agent in real life?  Gennaro Liccardi, MD, Antonello Salzillo, MD, Maria.
Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.

CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge  Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge  Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling  Jennifer Kearley, PhD, Douglas S. Robinson,

CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling  Jennifer Kearley, PhD, Douglas S. Robinson,
Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy  Meiqin Wang, MD, PhD, Ivana V. Yang,

Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy  Meiqin Wang, MD, PhD, Ivana V. Yang,
Differential roles for the IL-9/IL-9 receptor α-chain pathway in systemic and oral antigen–induced anaphylaxis  Heather Osterfeld, BSc, Richard Ahrens,

Differential roles for the IL-9/IL-9 receptor α-chain pathway in systemic and oral antigen–induced anaphylaxis  Heather Osterfeld, BSc, Richard Ahrens,
Nasal allergen provocation test with multiple aeroallergens detects polysensitization in local allergic rhinitis  Carmen Rondón, MD, PhD, Paloma Campo,

Nasal allergen provocation test with multiple aeroallergens detects polysensitization in local allergic rhinitis  Carmen Rondón, MD, PhD, Paloma Campo,
Experimental gastrointestinal allergy enhances pulmonary responses to specific and unrelated allergens  Eric B. Brandt, PhD, Troy A. Scribner, MD, Hiroko.

Experimental gastrointestinal allergy enhances pulmonary responses to specific and unrelated allergens  Eric B. Brandt, PhD, Troy A. Scribner, MD, Hiroko.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E

Similar presentations

Ads by Google