1. Abnormal methylation and total expression at IG-DMR in E10
Abnormal methylation and total expression at IG-DMR in E10.5 mKO offspring.
Abnormal methylation and total expression at IG-DMR in E10.5 mKO offspring. (A) The mouse IG-DMR locus. The maternal allele and maternal-specific Meg3 expression are represented at the top (pink) and the paternal allele and paternal-specific Dlk1 expression are represented at the bottom (blue). See Fig. 2 legend for details. (B) Percentage DNA methylation at IG-DMR. Embryos are in the same order from left to right as in Fig. 2D,E. mCON n=32 embryos from 4 litters, mKO n=36 embryos from 7 litters. **P<0.01, Fisher's exact test. (C) Total expression from qPCR analysis of Dlk1 and Meg3. All mCON litters and embryos are in the same order as in Fig. 2F. See Fig. 2 legend for details. In the Meg3 plot, expression in embryos A,D-L is undetectable.
Jennifer M. SanMiguel et al. Development 2018;145:dev160622
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