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Two domains of KHC act to position the nucleus.

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Presentation on theme: "Two domains of KHC act to position the nucleus."— Presentation transcript:

1 Two domains of KHC act to position the nucleus.
Two domains of KHC act to position the nucleus. Membranes are stained with fluorescent wheat germ agglutinin (red) in st9 wild-type (A) and Khc27 oocytes (GLCs) without (B) or with KHC-GFP transgenes (green in C-E). White line indicates the anterior membrane. (A) Wild-type nucleus positioning to the anterior-dorsal corner (arrow, note that the nucleus is in contact with the anterior membrane, but that the view axis is partially rotated). (B) In Khc null oocytes the nucleus is no longer attached to the anterior cortex. (C) An equivalent phenotype is observed in KHC1-700 oocytes. (D) Tailless KHC1-849 is able to weakly rescue nucleus positioning. (E) Full-length KHC fully rescues nucleus positioning. DAPI, blue. *P=0.088 (left) and *P=0.95 (right) for KHC1-700 versus KHC1-849 (Fisher’s exact test), considered to be not statistically significant. Lucy S. Williams et al. Development 2014;141: © Published by The Company of Biologists Ltd

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