1. FGF21 signaling in hypothalamus is blocked by FGFR1 neutralizing antibody.
FGF21 signaling in hypothalamus is blocked by FGFR1 neutralizing antibody. Twelve-week-old FGF21 KO mice were treated with an anti-FGFR1 monoclonal antibody (AF, 0.2 μg/g body weight) or mouse nonimmune IgG (NI) by a single hypothalamic paraventricular (intra-PVN) injection, followed by another intra-PVN injection with rmFGF21 (0.02 μg/g body weight) or vehicle control. Hypothalamic protein levels of p-ERK and t-ERK were determined by Western blotting analysis after 30 min of injection and were quantified using scanning densitometry. Representative Western blotting analysis from three independent cohorts run in duplicate are shown; n = 6. *P < Data were expressed as mean ± SEM.
Qingning Liang et al. Diabetes 2014;63:
©2014 by American Diabetes Association