1. NK cell function of spleen MNCs (A) and NK cell frequencies in the ileum, duodenum, and spleen (B) of protein-deficient and -sufficient HIFM/HRV-infected pigs.
NK cell function of spleen MNCs (A) and NK cell frequencies in the ileum, duodenum, and spleen (B) of protein-deficient and -sufficient HIFM/HRV-infected pigs. MNCs were isolated from the spleen, blood, ileum, and duodenum of piglets in the four experimental groups at PID14. (A) Spleen MNCs (piglets from all experimental groups) and carboxyfluorescein succinimidyl ester-stained K562 tumor cells were used as effector and target cells, respectively, and cocultured at set ratios to assess the cytotoxic function of NK cells (n = 10/group). Asterisks indicate statistically significant difference (two-way ANOVA; P ≤ 0.05). Error bars indicate the SEM. (B) NK cell frequencies among total MNCs were assessed by flow cytometry (n = 10/group). Asterisks indicate statistically significant differences (Kruskal-Wallis test; P ≤ 0.05). Error bars indicate the SEM.
Anastasia N. Vlasova et al. mSphere 2017; doi: /mSphere