1. Area-specific decrease of KCC2 immunofluorescent staining in slices exposed to the 0-Mg2+ solution.
Area-specific decrease of KCC2 immunofluorescent staining in slices exposed to the 0-Mg2+ solution. A, Slices superfused for 1 hr in control solution (Aa) and in the Mg2+-free solution (Ab). Note the pronounced reduction in staining intensity in CA1. so, Stratum oriens; sr, stratum radiatum; sl, stratum lucidum; slm, stratum lacunosum-moleculare; DG, dentate gyrus). B, Plot of optical intensities from the distinct areas marked in Aa and Ab after 1 and 3 hr of 0-Mg2+ superfusion. (t test; ***p < 0.001; n = 10.) Scale bar, 400 μm.
Claudio Rivera et al. J. Neurosci. 2004;24:
©2004 by Society for Neuroscience