1. Subcellular localization of prestin and mutant proteins in type I, II and III cells.
Subcellular localization of prestin and mutant proteins in type I, II and III cells. Immunofluorescence images of OK cells transiently transfected with wild type (wt-gPres) and three deletion mutants: Del516, Del709 and Del719. Three morphological phenotypes: types I, II and III are indicated for each mutant-expressing cell on the left side of the images. Red: anti-Golgi or anti-Na+/K+ ATPase markers. Na+/K+ ATPase is a plasma-membrane protein. Green: FITC-labeled anti-N-mPres, which indicates the location of prestin. Yellow images are superimposed from green and red images, indicating the co-localization of prestin and Na+/K+ ATPase or Golgi marker in the cell's plasma membrane or intracellular membranes. For better examination of the co-localization of prestin with the red markers, the images of the far right column are given at higher magnification. These segments correspond to the locations marked with white arrows. Note that in type II cells (example: Del709) the two labels are very close, but do not superimpose. Similarly, in some cells classified as type III, the two markers form parallel, adjacent, but not overlapping layers. In other cells, classified as type III (wild type and Del719), there is clear overlap, indicated by yellow color. Images were taken 48 hours after transfection. Bar, 20 μm.
Jing Zheng et al. J Cell Sci 2005;118:
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