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Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization  Julie M Clyde, Ph.D., Jan E Hogg, M.Sc., Anthony J Rutherford,

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1 Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization 
Julie M Clyde, Ph.D., Jan E Hogg, M.Sc., Anthony J Rutherford, M.B.B.S., Helen M Picton, Ph.D.  Fertility and Sterility  Volume 80, Issue 4, Pages (October 2003) DOI: /S (03)

2 FIGURE 1 M-FISH pseudocolor image of a normal oocyte metaphase (23,X) with (a) inverted DAPI stained chromosomes inset and (b) corresponding karyotype. (c and d), Hyperploid oocyte 24,X+18 showing nondisjunction, with the additional chromosome 18 indicated by an arrow. Pseudocolor image of (e) sperm prematurely condensed chromosomes in an injected oocyte (f) karyotyped as 23,X. All chromosomes are shown in M-FISH option to demonstrate probe specificity (flat color). Clyde. M-FISH in metaphase II oocytes.Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )


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