1. The conserved region upstream of wt1a is required for its expression in the intermediate mesoderm.
The conserved region upstream of wt1a is required for its expression in the intermediate mesoderm. (A) Schematic representation of wt1a reporter constructs. The upper row represents the plasmid pBS-wt1a-GFP, which was used for the generation of wt1a::GFP transgenic zebrafish. In addition, two truncated wt1a-GFP plasmids are shown containing a 6.8 kb genomic fragment (middle row) and the same fragment lacking 100 bp within the conserved region (lower row). A detailed illustration of the deleted 100 bp region is shown in Fig. 5B. (B) Overlay of brightfield transmission and fluorescence images from wt1a::GFP (left) and wt1a-6.8kb::GFP (right) transgenic embryos at 17 and 42 hpf (top). For detailed analysis, embryos at 17 hpf were stained with anti-GFP antibody (green) and were sectioned (bottom). Counterstaining with DAPI is shown in false color (red). gl, glomeruli; im, intermediate mesoderm.
Frank Bollig et al. Development 2009;136:
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