1. Genetics of Brain Development and Hydrocephalus
Biomedical RaMP Final Presentation
April 19, 2016
Genetics of Brain Development and Hydrocephalus
Scott Emmert
Mentor/PI: June Goto, PhD

2. The Research Mangano Research Lab, Department of Neurosurgery, CCHMC
Dr. June Goto: PI, Research Instructor, and Neurobiologist
Pediatric hydrocephalus – “water on the brain”
New mutant gene - “progressive hydrocephaly, prh”
Rat-prh project to study role of this gene in brain development

3. What? DNA Work Tissue Culture CRISPR/cas9 genome editing
Preparing/splitting cells
Plasmid preparation
Lipofection
Bacteria transformation
Electroporation
Gel electrophoresis
DNA Sequencing & Genotyping Core
PCR

4. Positive sequencing samples
What? - Timeline
Positive sequencing samples
Transfection
Rat work, surgery

5. So What? Sense of fulfillment Lab-to-life skills
Gradually making a difference in lives of pediatric hydrocephalus patients
Lab-to-life skills
Accountability
Collaboration
Experiential learning
Self-efficacy

6. Now What? Continuation of research into summer through SURF
Further pursue rat-prh project
Engage research as an application of my coursework
Self-discovery
Completely new perspective on biomedical research
Career possibilities
Physician-scientist