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Lack of Membrane Expression of Interleukin-2 Receptor α Chain (CD25) in Mycosis Fungoides: Application of Laser Scanning Cytometry for Phenotyping of Skin Infiltrating Lymphocytes Robert Gniadecki Journal of Investigative Dermatology Volume 114, Issue 3, Pages (March 2000) DOI: /j x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 1 Immunophenotyping of lesional lymphocytes from a patient with mycosis fungoides. The viable lesional lymphocytes (A, B) were labeled as indicated in the text and scanned. The isotype control staining in (A) defines the background fluorescence. In (C), the cells were permeabilized and restained with the antibodies to detect the cytoplasmic antigen. In this case, the cytoplasmic, but not the membrane expression of CD25 is seen. Journal of Investigative Dermatology , DOI: ( /j x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions
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