1. RIPA-based buffer system Harsh-conditioned system
Supplementary Fig. S5 a
a b c Uev1a E2 (UbcH)
FLAG-Ub E1
GST
GST-ZNF313-WT
GST-ZNF313-Ub(n)
 GST-ZNF (Expected size)
170 -
72 -
55 -
IB: anti-FLAG b
170 -
72 -
55 -
PBS a b c E2 (UbcH)
FLAG-Ub + E1
GST-ZNF313-WT
GST-ZNF313 RING-MT
GST-ZNF313 ΔUIM
GST-ZNF313-Ub(poly)
 GST-ZNF (Expected size)
 GST-ZNF313-Ub(mono)
IB: anti-FLAG c
100 -
55 -
35 -
WCL
HeLa
HA-Ub
siZNF313-1
RIPA-based buffer system
Harsh-conditioned system
IP: p21WAF1
IB: HA
IB: p21WAF1
170 -
ZNF313
p21WAF1 HA
Tubulin