1. MLST analysis of Bacillus strains of this study and related species of the B. cereus sensu lato group.
MLST analysis of Bacillus strains of this study and related species of the B. cereus sensu lato group. The genomic contigs of ISS isolates obtained were searched for glpF, gmk, ilvD, pta, pur, pycA, and tpi gene sequences, which are standardized for use in MLST of the B. cereus sensu lato group of species (43). The sequences retrieved were compared with the STs deposited in the B. cereus MLST database (44), concatenated according to the MLST scheme. It was found that all 11 ST sequences derived from the ISS isolates are identical and do not correspond to any known allele combination. The reconstruction was based on the RAxML algorithm (45), and the bootstrap values were calculated by using 500 replicates. The bar indicates 1% sequence divergence.
Kasthuri Venkateswaran et al. mSystems 2017; doi: /mSystems