Presentation is loading. Please wait.

Presentation is loading. Please wait.

Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly  Raffaela Campana, PhD, Susanne Vrtala,

Published bySandra Patterson Modified over 5 years ago

Similar presentations

Presentation on theme: "Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly  Raffaela Campana, PhD, Susanne Vrtala,"— Presentation transcript:

1 Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly  Raffaela Campana, PhD, Susanne Vrtala, PhD, Bernhard Maderegger, PhD, Peter Jertschin, PhD, Gottfried Stegfellner, PhD, Ines Swoboda, PhD, Margarete Focke-Tejkl, PhD, Katharina Blatt, Anna Gieras, MSc, Domen Zafred, BSc, Angela Neubauer, PhD, Peter Valent, MD, Walter Keller, PhD, Susanne Spitzauer, MD, Rudolf Valenta, MD  Journal of Allergy and Clinical Immunology  Volume 126, Issue 5, Pages e8 (November 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Hypoallergenic rBet v 1 derivatives. A, Construction scheme of the rBet v 1 derivatives. The amino acids at the borders of the protein segments and the binding sites of 2 mAbs, mAb#2 and mAb#12, are indicated. B, SDS-PAGE of purified rBet v 1 and rBet v 1 derivatives. M, Molecular mass markers (kd). Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 IgG reactivity of rBet v 1 and rBet v 1 derivatives with Bet v 1–specific antibodies. Nitrocellulose-blotted rBet v 1 and rBet v 1 derivatives were probed with rabbit anti–Bet v 1 antibodies (rαBet v 1) (A), mAb Bip 1 (B), mAb#2 (C), or mAb#12 (D). Molecular weights are displayed in the right margins in kilodaltons. M, Marker. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 IgE reactivity of rBet v 1 and rBet v 1derivatives. Nitrocellulose-dotted rBet v 1, rBet v 1-rs1, rBet v 1-rs2, rBet v 1-mosaic, HSA, and BSA were exposed to sera from patients with birch pollen allergy (1-19), individuals without allergy (20-21), buffer (0), or anti–rBet v 1 antibodies (22). Bound IgE and rabbit IgG antibodies were detected and visualized by autoradiography. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Allergenic activity of rBet v 1 and rBet v 1 derivatives as determined by CD203c upregulation. Blood samples from birch-allergic patients (A-F) were exposed to increasing concentrations (0.005 to 50 pmol/L) of rBet v 1, an equimolar mixture of rBet v 1 fragments (F1+F2) or rBet v 1-rs1 (x-axes; A), and in additional patients (G-J) with rBet v 1, rBet v 1-rs1, rBet v 1-rs2, and rBet v 1-mosaic (x-axes; B). Anti-IgE served as a positive control. The stimulation indices (SI; y-axes) reflect the upregulation of CD203c expression compared with buffer (0). Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Gel filtration profiles of rBet v 1 (A), Bet v 1-rs2 (B), Bet v 1-mosaic (C), and Bet v 1-rs1 (D). The x-axes show the elution volumes (mL), and the y-axes the adsorbance of the proteins at 280 nm. Arrows indicate the approximate molecular masses (kd) as determined with the standards. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Allergenic activity of rBet v 1 and rBet v 1 derivatives as determined by CD203c upregulation. Blood samples from 3 patients with birch allergy (K-M) were exposed to increasing concentrations ( pmol/L) of rBet v 1, rBet v 1-rs1, rBet v 1-rs2, and rBet v 1-mosaic (x-axes). Anti-IgE served as a positive control. The mean stimulation indices (SIs; y-axes) reflect the upregulation of CD203c expression compared with buffer (0). SDs are indicated. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Reactivity of IgG antibodies with rBet v 1 and rBet v 1 derivatives
Reactivity of IgG antibodies with rBet v 1 and rBet v 1 derivatives. Different dilutions (x-axes) of rabbit anti–rBet v 1 (anti–Bet v 1: ●), anti-rBet v 1 derivative antibodies (anti–Bet v 1-rs1: ■; anti–Bet v 1-rs2: ▴; anti–Bet v 1-mosaic: x) or antibodies from a normal rabbit (—) were reacted with rBet v 1 (A), rBet v 1-rs1 (B), rBet v 1-rs2 (C), or rBet v 1-mosaic (D). OD values (y-axes) correspond to the amounts of bound antibodies. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Reactivity of rabbit antibodies raised with aluminum hydroxide–adsorbed proteins by ELISA. Different dilutions (x-axes) of rabbit anti–rBet v 1 (anti–Bet v 1: ●), anti–rBet v 1—derivative antibodies (anti–Bet v 1-rs1: ■; anti–Bet v 1-rs2: ▴ anti–Bet v 1-mosaic: x) or antibodies from a normal rabbit (—) reacted with rBet v 1. OD values (y-axes) correspond to the amounts of bound antibodies. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly  Raffaela Campana, PhD, Susanne Vrtala,"

Similar presentations

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,
Natural latex, grass pollen, and weed pollen share IgE epitopes

Natural latex, grass pollen, and weed pollen share IgE epitopes
Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines 

Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines 
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Identification of common allergenic structures in mugwort and ragweed pollen  Reinhold Hirschwehr, MDa, Claudia Heppner, MSca, Susanne Spitzauer, MDb,

Identification of common allergenic structures in mugwort and ragweed pollen  Reinhold Hirschwehr, MDa, Claudia Heppner, MSca, Susanne Spitzauer, MDb,
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Identification and immunologic characterization of an allergen, alliin lyase, from garlic (Allium sativum)  Shao-Hsuan Kao, MS, Ching-Hsian Hsu, MD, PhD,

Identification and immunologic characterization of an allergen, alliin lyase, from garlic (Allium sativum)  Shao-Hsuan Kao, MS, Ching-Hsian Hsu, MD, PhD,
Asian ladybugs (Harmonia axyridis): A new seasonal indoor allergen

Asian ladybugs (Harmonia axyridis): A new seasonal indoor allergen
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin) 

Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin) 
Frequent occurrence of T cell–mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments  Raffaela Campana, PhD, Katharina.

Frequent occurrence of T cell–mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments  Raffaela Campana, PhD, Katharina.
Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.
Determination of allergen specificity by heavy chains in grass pollen allergen–specific IgE antibodies  Elisabeth Gadermaier, PhD, Sabine Flicker, PhD,

Determination of allergen specificity by heavy chains in grass pollen allergen–specific IgE antibodies  Elisabeth Gadermaier, PhD, Sabine Flicker, PhD,
Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.
Natural latex, grass pollen, and weed pollen share IgE epitopes

Natural latex, grass pollen, and weed pollen share IgE epitopes
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule  Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule  Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,
Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Similar presentations

Ads by Google