1. Fig. 2. The Clu and TPR domains are required for Clu function in vivo.
The Clu and TPR domains are required for Clu function in vivo. (A) Expressing UAS-clu domain deletion constructs in a clu null background fails to rescue the delay in pupation. Dashed lines represent larvae expressing each deletion construct under the control of daGAL4 (clud08713; daGAL4/UAS-transgene). The color-matched solid lines represent the wild-type siblings as a control (clud08713/CyO ActGFP; daGAL4/UAS-transgene). clud08713 (orange) and clud08713; daGAL4/UAS-cluh (white) are negative and positive controls for pupation rate, respectively. (B) UAS-driven Clu constructs missing each putative domain fail to rescue adult survival when ectopically expressed in a clu mutant background using daGAL4. (C) Survival of clud08713; daGAL4/UAS-cluh adults stabilizes after a week, and the remaining flies live a normal lifespan (orange line). y w (grey) and clud08713; daGAL4/UAS-FL-clu (black) are positive controls and clud08713 (white) is a negative control. (D) Adult ATP levels normalized to clu heterozygous flies. Adult flies over-expressing full-length Clu (FL) using daGAL4 have significantly increased ATP levels compared to clu null adult flies. Adult flies over-expressing ΔDUF, ΔBGF, ΔClu and ΔTPR using daGAL4 do not show any increase in ATP levels. Error bars indicate standard error of mean. P-values were calculated in Excel using a two-tailed Student's t-test. **P<0.008, ***P<0.0006, ****P<
Aditya Sen, and Rachel T. Cox Biology Open 2016;5:
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