1. Loss of ezrin and MISP leads to the formation of cysts containing multiple lumens and spindle orientation defects.
Loss of ezrin and MISP leads to the formation of cysts containing multiple lumens and spindle orientation defects. Three-dimensional reconstitution of Caco-2 BBe cysts transfected with the indicated siRNAs. Caco-2 BBe cysts were stained with antibodies directed against MISP (magenta) and ezrin (green) (A) or with antibodies against α-tubulin (green) and pericentrin (PCNT, magenta) (B), and co-stained with Hoechst (blue, DNA). Maximum projections of three confocal z-stacks through the middle region of the cysts are shown. Scale bars: 5 µm. In A, the quantification shows the percentage of cells with single (normal) or multiple lumens, as indicated in the illustration. Data represent mean±s.d. from three independent experiments, n>102 cells per experiment. In B, the quantification shows the spindle angle formed from the middle of the spindle and the mid point of the cyst. Data represent mean±s.d. from three independent experiments, n>59 cells. **P<0.01, ***P<0.001, ****P< (unpaired Student's t-test).
Yvonne T. Kschonsak, and Ingrid Hoffmann J Cell Sci 2018;131:jcs214544
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