1. Immunofluorescence analysis of GpGCS1 localization in strains K41 (mating-type plus) and K34 (mating-type minus).
Immunofluorescence analysis of GpGCS1 localization in strains K41 (mating-type plus) and K34 (mating-type minus). (A) Unactivated minus and plus gametes were stained with anti-GpGCS1 antibody. The fluorescence images are maximum-intensity projections from confocal z-stacks. Images formed by merging the fluorescence and differential interference contrast (DIC) images are also shown. Asterisks and arrowheads indicate flagella and their roots, respectively. Scale bars, 5 μm. (B) minus and plus gametes activated by db-cAMP and IBMX treatment were double stained with anti-GpGCS1 and anti-actin antibodies. The merged and phase-contrast images are also shown. Arrowheads indicate the tips of the TMSs. Scale bars, 5 μm.
Hiroko Kawai-Toyooka et al. Eukaryotic Cell 2014; doi: /EC