1. Mesothelial cell–associated hyaluronic acid promotes adhesion of endometrial cells to mesothelium 
Herve Dechaud, M.D., Craig A. Witz, M.D., Iris A. Montoya-Rodriguez, B.S., Linda A. Degraffenreid, Ph.D., Robert S. Schenken, M.D. 
Fertility and Sterility 
Volume 76, Issue 5, Pages (November 2001)
DOI: /S (01)

2. Figure 1
Dose response of hyaluronidase treatment of mesothelial cells prior to plating endometrial stromal cells (ESCs) (n = 2) and endometrial cells (EECs) (n = 2). Binding to mesothelial cells treated with hyaluronidase is compared to binding to untreated mesothelium. Maximimum inhibition of binding was seen at hyaluronidase concentrations of 2.5–5.0 mg/mL.
Dechaud. Mesothelial hyaluronate and endometrial cell adhesion. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

3. Figure 2
Effect of hyaluronidase treatment of mesothelial cells prior to plating ESCs and EECs. In the ESC assay, there was 49.5% binding to untreated mesothelium (grey bars) compared to 30.0% binding to hyaluronidase treated mesothelium (white bars) (P<.02). In the EEC assay, there was 40.6% binding to untreated mesothelium (grey bars) compared to 28.1% binding to hyaluronidase treated mesothelium (white bars) (P<.03). (Error bars = SEM).
Dechaud. Mesothelial hyaluronate and endometrial cell adhesion. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

4. Figure 3
Effect of hyaluronidase treatment of ESCs and EECs prior to plating on untreated mesothelium and collagen IV. Compared to untreated cells (grey bars), there was no difference in the binding of hyaluronidase treated (white bars) ESCs or EECs to mesothelium or collagen IV. (Error bars = SEM)
Dechaud. Mesothelial hyaluronate and endometrial cell adhesion. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

5. Figure 4
Western blot of CD44s. There was strong expression of CD44s by the ovarian cancer cells (SKOV-3), which served as a positive control. There was a variable degree of expression in endometrial epithelial cells from different patients (EEC 1 and EEC 2). Likewise, there was a variable degree of expression in endometrial stromal cells from different patients (ESC 1 and ESC 2). There was no expression of CD44s in platelets (negative control).
Dechaud. Mesothelial hyaluronate and endometrial cell adhesion. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )