1. The coreceptor programmed death 1 inhibits T-cell adhesion by regulating Rap1 
Inbar Azoulay-Alfaguter, PhD, Marianne Strazza, PhD, Ariel Pedoeem, BS, Adam Mor, MD, PhD 
Journal of Allergy and Clinical Immunology 
Volume 135, Issue 2, Pages e1 (February 2015)
DOI: /j.jaci
Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2. Fig 1
PD-1 inhibits T-cells adhesion. Jurkat and primary T cells were treated as indicated and adhesion to ICAM-1 (A) and Rap1 activation (B) was measured. Cells expressing the probe for activated Rap1 were treated as indicated and imaged alive (C). D, Jurkat T cells treated as indicated were cocultured with Raji B cells and percentages of transient and stable conjugates were recorded. GTP, Guanosine-5′-triphosphate; SEE, Staphylococcus enterotoxin E. *P < .05.
Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci )
Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3. Fig 2
PD-1 inhibits C3G phosphorylation and Rap1 activation. T cells were treated as indicated and levels of phospho-C3G were measured in Jurkat (A and C) and primary (B) T cells. T cells were treated as indicated and levels of activated Rap1 (D) and adhesion to ICAM-1 (E) were measured. F, Model to explain the effects of PD-1 on T-cell adhesion. GTP, Guanosine-5′-triphosphate; LFA-1, lymphocyte function-associated antigen-1. *P < .05. **Not significant.
Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci )
Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions